The double Olympic gold medal victor, who clinched gongs at the 2012 and 2016 Games in London and Rio respectively, had been set to fight on the undercard of the middleweight world title showdown between Saul Alvarez and Gennady Golovkin.
She tweeted: "I'm devastated that I'm not boxing tonight, due to a problem with my opponent, thank you everyone for your support and kind messages".
Nicola Adams had already arrived at the arena when she discovered that her debut in the United States was to be scrapped.
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Vlajk failed her pre-fight medical, meaning that the fight with Leeds' Adams had to be pulled from the bill.
She told the BBC earlier in the week: "This is something you dream about as a kid". "It may never come true and it's nice to think I'm able to achieve my dreams".
"I don't know if we've ever had women on the undercard of such a big event before, or in Vegas". We are definitely changing the game for the better'.
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